Genomic Engineering Group / InteLAB
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Tuesday, 14 July 2020
Genetic Engineering
Genetic engineering (or recombinant DNA technology) is a key technology in our studies. We particularly committed to develop biomedical applications using DNA sequence publicly available so we can use bioinformatics and metabolic engineering tools to advance biomaterial properties useful for tissue engineering applications. We call this approach “molecular tissue engineering” since it relies on molecular information to acquire, design or develop novel material (macroscopic) properties. So far we are at initial stages of what promises to be a very fruitful research field.

  • C. violaceum  ( 3 items )

    Chromobacterium violaceum is a remarkable microorganism. The C. violaceum ATCC 12472 genome was sequenced by The Brazilian National Genome Project Consortium based on its potential for biotech and pharmaceutical applications.

    Based on the sequenced genome we generated the pathway-genome database (PGDB) called CvioCyc ( that is now being used to investigate metabolic routes, regulatory controls and gene structures, particularly operon organization.

    C. violaceum has been used in our group as a model organism to study several biological functions, including:

    * Intercelular communication (Quorum sensing)

    * Antitumoral production (Violacein biosynthesis)

    * Biopolymer production (PHAs and cellulose)

    * Bacterial infections (Pathogenicity) and antibiotics

    * Operon organization

    * Polymerization active sites of bacterial enzymes

    Knowledge from the C. violaceum studies is then used to clone genes and operons into other species (E. coli) to heterologously produce potential biomedical products.

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